Technologist - Vaccines

Reference No. R2741420

Position Title: Technologist- Vaccines

Duration: Fixed-Term - Contract End Date: May 31, 2027

Department: B100 DT (Days)

Location: Toronto, Ontario

Sanofi Vaccines

Sanofi's global vaccines business unit partners with the public health, medical and scientific communities to improve access to life-protecting vaccines and increase vaccination coverage, while striving to develop new and improved vaccines to enhance health and well-being.

At Sanofi, we chase the miracles of science to improve people's lives. We believe our cutting-edge science and manufacturing, fueled by data and digital technologies, have the potential to transform the practice of medicine, turning the impossible into possible for millions of people.

• Primaryand Secondary (P&S) seed expansion, purity testing, gram stain, evaluationand recovery of Tetanus and Diphtheria cultures for large scale fermentationapplications.
  
• Prepare and load lyophilizer for different seed lines for master and working seed stock. Separation and heat sealing the lyophilized cultures. Evaluation on generated seed stocks for commercial licensed applications.
  
• Prepare raw-reagents intermediate reagents and intermediate products for seed preparation. Final test and initial primary release of final stage of seed culture for inoculation into fermenters.
  
• P & S propagation of working seed lines for process improvement or development applications.
  
• Post verification/qualification of manufactured seed lines conduct requires testing, obtain Raw Material specifications sheet and submit to QA/RA for culture release.
  
• Complete appropriate documentation and appropriate release.
  

• Cleaning fermentors and related equipment, rinsing and preparation of fermentation equipment for sterilization and fermentation of batches. Release of equipment for further processing.
  
• Create, amend and update SOPs to perform necessary steps to formulate products. Where needed set-up, monitor and manipulate control towers to maintain or modify specific fermentation parameters. Maintenance of the SPC data based for detailed data compilations.
  
• Aseptically sample fermentation batch, perform and release purity test, and evaluate culture to continue fermentation or processing. A critical understanding of the fermentation process, by- products and integrity measures. Proper documentation on the associated controlled documents.
  
• Ensure chemical calculations, chemical additions and elemental requirements are met for the different fermentations. Preparations of solutions, dilutions and in-process diluents for products. Strong understanding of dilutions and chemical compositions in solutions.
  
• Trouble shooting problems arising within processes. Strong understanding of
  
• fermentors, fermentor design, equipment integrity and testing (including pre
  
• testing).
  
• Batch clean- p and tum-over.
  

• Prepare chemical and equipment resources for harvesting of fermented batches.
  
• Set-up of crude filtration units, ultra-filtration unit, and equipment for recovery, purification, concentration and detoxification of fermented batches.
  
• Monitor,maximize, manipulate and document parameters for the recovery processing.
  
• Filter and sterilize product, concentrate and purify products, then performdetoxification of toxins.
  
• Evaluate primary release criteria for process continuation. Perform primary and secondar recoveries of toxins. Evaluate for processing criteria.
  
• Strong understanding of filters and filtration. Strong understanding of commercial I product recovery equipment and protein recovery techniques.
  

• Perform all required purity testing of Diphtheria and Tetanus during seedpropagation, master & working seed creation, fermentation, and harvesting.
  
• Evaluate and document purity of product prior to continuation of batch.
  
• Prepare and deliver QC release MLD/L+ testing and required TRs. Receive results and evaluate.
  
• Perform Specifies toxicity and sterility post tetanus incubation.
  

• Obtain and verify required material for EM and utilities testing.
  
• Monitor and perform aseptic EM required per product/per week, bi-weekly and monthly schedules.
  
• Install, prepare, and flush chilling unit/air sampling unit for proper sampling application.
  
• SterilizeI/P.S. lines, monitor and aseptically sample.
  
• Aseptically perform required WFI/steam/compressed air sampling as per schedule.
  
• Assistin SIR and EIR reports, assist in product testing if any.
  
• Fillout required documentation and ensure that samples are delivered on time to theirintended departments.
  

• Review SOPs every three years and update. Create new SOP if needed.
  
• Receive updated SOPs and update SOP binders.
  
• Ensure departmental training of new or revised SOP., support, amend and analyze data bases.
  
• Montior, support, amend and analyze data bases.
  
• Maintain current data bases.
  

• Minimum three (3) years Community College Diploma (Microbiology, Biochemistry, Biotechnology), plus a minimum of two (2) years of previous relevant work experience.
  
• Preferred three (3) years University degree (Microbiology/Biology, Biochemistry, Protein Research), plus a minimum of one (1) year of previous relevant work experience.
  
• Microbiological Understanding- growth kinetics
  
• Aseptic techniques
  
• Mechanical and processing techniques
  
• Chemistry
  
• Statistical, Analytical/Deductive reasoning
  
• Protein Purification
  
• Mathematics
  
• Microbiology
  
• Coordinating, organization, verbal and written communication skills
  

• Inclusive from Monday to Friday: 2:00 p.m. - 10:00 p.m.