Supervisor, Research Laboratory

External Description

The Immunotherapy Integration Hub (IIH) at Seattle Children’s Research Institute (SCRI) seeks to develop innovative new cellular therapies and translate these advances to groundbreaking clinical trials for children with the most aggressive forms of cancer. We are seeking a bright, enthusiastic, and highly-motivated laboratory supervisor to join the Research and Development Group. This position contributes to a team focused on translating tumor immunotherapy from the bench into pediatric patients with cancer. We look for people who are inspired by our mission and who would fit in well with the collaborative, rigorous, and entrepreneurial spirit of our research team culture.

 

The primary purpose of this position is to help coordinate the programmatic activities and needs of the research and development program in compliance with Good Laboratory Practice (GLP) standards. Under the direction of a senior research scientist, the successful candidate will train personnel and implement and maintain GLP standards within the lab.

 

The ideal candidate will also have work experience in molecular biology, immunology, tissue culture and flow cytometry. The candidate will help train personnel in standard laboratory techniques, prepare reports, write/review Standard Operating Procedures (SOPs), maintain budgets, and manage equipment in support of funded research studies. To ensure successful project completion and the maintenance of GLP compliance, she/he must have critical thinking abilities, be able to prioritize and perform multiple tasks in a dynamic environment, meet deadlines, and must have excellent written, verbal and interpersonal communication skills. The candidate will be required to participate and present data in weekly lab meetings and maintain an up-to-date and well-organized electronic lab notebook.

Requirements

Required Education/Experience:
- Bachelor's Degree in Cellular/Molecular Biology, or related field
- Minimum of five (5) years laboratory research experience (type of experience may vary based on lab needs), to include experience with laboratory management, LIMS, and GLP/cGMP.
- Experience with a broad range of techniques for analyzing cell function and molecular biology.

Preferred:

• At least 2 years of hands-on experience in molecular and cellular biology techniques; protein, DNA and RNA methods including but not limited to Western-blot analysis, recombinant DNA technology, PCR (including primer design, qPCR, and ddPCR), bacterial culture.

• Expert knowledge in molecular cloning including restriction cloning, Gibson cloning, construct design, a working knowledge/experience with droplet digital PCR, CRISPR technologies, and single cell RNA sequencing technologies
• Experience culturing adherent and suspension mammalian cells and the transient transfection or lentiviral transduction of cell lines and primary human T cells
• Experience in multi-color flow cytometry and plate based assays such as cytokine release and cytotoxicity assays.
• Experience in assay development, execution, analysis, and troubleshooting
• Experience working in a Good Laboratory Practice (GLP) regulated environment and understanding of safe lab practices and BSL requirements.
• Excellent computer skills and extensive working knowledge of Microsoft Office applications, Vector NTI, NCBI tools, ELN, FlowJo, GraphPad.
• Able to effectively prioritize work assignments and address competing demands of multiple projects as part of a collaborative research team


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