Research Scientist II
The Ben Towne Center for Childhood Cancer Research (BTCCCR) at Seattle Children's Research Institute (SCRI) seeks to develop innovative new cellular therapies and translate these advances to groundbreaking clinical trials for children with the most aggressive forms of cancer. We are seeking bright, enthusiastic, highly-motivated and experienced molecular and/or cellular biologists to join the Jensen Lab at BTCCCR. This position contributes to a team focused on translating tumor immunotherapy from the bench into pediatric patients with cancer. We look for people who are inspired by our mission and who would fit in well with the collaborative, rigorous and entrepreneurial spirit of our research team culture.
The primary purpose of this position is the development and testing of chimeric antigen receptors (CARs) for use as clinical therapies. Under the direction of a senior research scientist, the successful candidate will use her/his established molecular/cellular biology and in vitro biological skill sets to construct lentiviral vectors and other DNA-based delivery platforms to create primary T cell lines which express and control CARs and CAR-related therapies, and evaluate them for biological and on-target activities. The candidate will prepare and modify DNA constructs in expression vectors and generate high-titer lentiviral vectors for transduction of T-cells to recognize and destroy cancer cells.
The ideal candidate will have significant work experience in molecular biology, immunology, tissue culture and flow cytometry. Of utmost importance, the candidate will have extensive knowledge of molecular cloning, PCR techniques, assay development, and state-of-the-art molecular platforms. The candidate will be extensively involved in designing experiments, implementing new strategies, generating data, analyzing results and preparing reports, in support of funded research studies. She/he must have critical thinking abilities, be able to prioritize and perform multiple tasks in a dynamic environment, meet deadlines, and must have excellent written, verbal and interpersonal communication skills, to ensure successful project completion and teamwork. The candidate will be required to participate and present data in weekly lab meetings and maintain up-to-date and well-organized electronic lab notebook.
This position level (II-III) will be contingent upon final candidate's background and qualifications as it relates to position needs.
- Bachelor's Degree in biology, microbiology, immunology, molecular biology or biochemistry. May require a Master's or PhD degree for certain lab specialties
- Dependent upon position a minimum of two (2) years experience in a related area/laboratory setting
- Significant individual initiative and motivation to research and learn scientific methods and knowledge in their project area
- Able to effectively prioritize work assignments and address competing demands of multiple projects as part of a collaborative research team
- Master's or PhD degree in applicable field/research specialty highly preferred
- At least 3 years of hands-on experience in molecular and cellular biology techniques; protein, DNA and RNA methods including but not limited to Western-blot analysis, recombinant DNA technology, PCR (including qPCR and RT-qPCR and primer design), bacterial culture.
- Expert knowledge in molecular cloning including restriction cloning, Gibson cloning, construct design and a working knowledge/experience with droplet digital PCR and CRISPR technologies
- Experience culturing adherent and suspension mammalian cells and the transient transfection or lentiviral transduction of cell lines and primary human T cells
- Strong organizational skills, including the ability to effectively prioritize work across projects
- Able to flex work schedule to meet laboratory timeline demands
- Experience in lentiviral vector design and production
- Experience in multi-color flow cytometry and plate based assays such as cytokine release and cytotoxicity assays.
- Experience in assay development, execution, analysis, and troubleshooting
- Experience working in a Good Laboratory Practice (GLP) regulated environment and the development of Standard Operating Procedures (SOP)
- Magnetic column based primary T cell and tumor cell isolation
- Excellent computer skills and extensive working knowledge of Microsoft Office applications, Vector NTI, NCBI tools, ELN, FlowJo, GraphPad.
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