Research Scientist II
The Immunotherapy Integration Hub (IIH) at Seattle Children’s Research Institute (SCRI) seeks to develop innovative new cellular therapies and translate these advances to groundbreaking clinical trials for children with the most aggressive forms of cancer. We are seeking a bright, enthusiastic, highly-motivated and experienced molecular and cellular biologists to join the Jensen Lab. This position contributes to a team focused on translating tumor immunotherapy from the bench into pediatric patients with cancer. We look for people who are inspired by our mission and who would fit in well with the collaborative, rigorous and entrepreneurial spirit of our research team culture.
The primary purpose of this position is to help develop transgenes that are designed to enhance chimeric antigen receptor T cells with the goal of making better clinical therapies. Under the direction of a senior research scientist, the successful candidate will use her/his established molecular/cellular biology skill sets to construct lentiviral vectors and create primary T cell lines which co express CARs and transgene, and evaluate them for biological and on-target activities.
The ideal candidate will have significant work experience in molecular biology, immunology, tissue culture and flow cytometry. The candidate will be extensively involved in designing experiments, implementing new strategies, generating data, analyzing results and preparing reports, in support of funded research studies. To ensure successful project completion and teamwork, she/he must have critical thinking abilities, be able to prioritize and perform multiple tasks in a dynamic environment, meet deadlines, and must have excellent written, verbal and interpersonal communication skills. The candidate will be required to participate and present data in weekly lab meetings and maintain up-to-date and well-organized electronic lab notebook.
- Bachelor’s Degree in an engineering or scientific discipline; may require a Master’s Degree for certain lab specialties
- Minimum to two (2) year’s experience in a related area/laboratory setting.
• At least 2 years of hands-on experience in molecular and cellular biology techniques; protein, DNA and RNA methods including but not limited to Western-blot analysis, recombinant DNA technology, PCR (including primer design, qPCR, and ddPCR), bacterial culture.
• Expert knowledge in molecular cloning including restriction cloning, Gibson cloning, construct design, a working knowledge/experience with droplet digital PCR, CRISPR technologies, and single cell RNA sequencing technologies
• Experience culturing adherent and suspension mammalian cells and the transient transfection or lentiviral transduction of cell lines and primary human T cells
• Experience in multi-color flow cytometry and plate based assays such as cytokine release and cytotoxicity assays.
• Experience in assay development, execution, analysis, and troubleshooting
• Experience working in a Good Laboratory Practice (GLP) regulated environment and understanding of safe lab practices and BSL requirements.
• Excellent computer skills and extensive working knowledge of Microsoft Office applications, Vector NTI, NCBI tools, ELN, FlowJo, GraphPad.
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