Research Specialist - Membrane Protein Labeling, Imaging and Functional Characterization

Summary:

The Research Specialist will develop high-throughput methods to label and image nanometer-scale structure of membrane-associated proteins (e.g. Ion channels and GPCR receptors) in the brain. The ultimate goal is to characterize the sub-cellular localization and dynamics of cell-type specific channel and receptor proteins in the brain and study how neural computation in single neuron units is regulated at the molecular level.

Essential Duties and Responsibilities:

  • Optimize high-throughput genome-editing methods to tag and label endogenous membrane proteins in the brain (mouse).
  • Design and perform electrophysiology experiments to validate the function of the tagged protein in cultured cells or in brain slices.
  • Collaborate closely with imaging tool builders at Janelia to develop super-resolution and electron microscopy pipeline to map and annotate the sub-cellular localization of membrane proteins in the brain.
  • Present data in group meetings, at conferences, and in publications
  • Perform other related duties as required


Scope:
  • No managerial responsibilities


Preferred Qualifications:
  • Ph.D. in Neuroscience, Molecular Biology, or related discipline
  • 3-5 years' experience with electrophysiology, imaging and/or stereotaxic virus injection techniques
  • Demonstrated experience in characterizing the function of particular channel or receptor proteins
  • Experience with fluorescence and/or electron microscopy
  • Data analysis skills
  • Excellent communication skills


Skills and Abilities:
  • Ability to perform extensive hands-on work at the bench, with minimal supervision
  • Ability to fully document and substantiate scientific methods, procedures, and results
  • Ability to manage and analyze large data sets


Physical Requirements:
  • Occasionally lifts and/or carries objects weighing up to 20 pounds
  • Occasionally stoops and bends
  • Occasionally reaches and grasps with arms and hands
  • Work involves possible exposure to laser sources, malodorous vapors, animal allergens and other animal-borne hazards, viruses, experimental microbial agents, low dose radiation, contamination by toxic chemicals and acids and presence of carcinogenic substances
  • Long periods of standing and/or sitting


HHMI is an Equal Opportunity Employer.


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