Research Specialist - Membrane Protein Labeling, Imaging and Functional Characterization
The Research Specialist will develop high-throughput methods to label and image nanometer-scale structure of membrane-associated proteins (e.g. Ion channels and GPCR receptors) in the brain. The ultimate goal is to characterize the sub-cellular localization and dynamics of cell-type specific channel and receptor proteins in the brain and study how neural computation in single neuron units is regulated at the molecular level.
Essential Duties and Responsibilities:
- Optimize high-throughput genome-editing methods to tag and label endogenous membrane proteins in the brain (mouse).
- Design and perform electrophysiology experiments to validate the function of the tagged protein in cultured cells or in brain slices.
- Collaborate closely with imaging tool builders at Janelia to develop super-resolution and electron microscopy pipeline to map and annotate the sub-cellular localization of membrane proteins in the brain.
- Present data in group meetings, at conferences, and in publications
- Perform other related duties as required
- No managerial responsibilities
- Ph.D. in Neuroscience, Molecular Biology, or related discipline
- 3-5 years' experience with electrophysiology, imaging and/or stereotaxic virus injection techniques
- Demonstrated experience in characterizing the function of particular channel or receptor proteins
- Experience with fluorescence and/or electron microscopy
- Data analysis skills
- Excellent communication skills
Skills and Abilities:
- Ability to perform extensive hands-on work at the bench, with minimal supervision
- Ability to fully document and substantiate scientific methods, procedures, and results
- Ability to manage and analyze large data sets
- Occasionally lifts and/or carries objects weighing up to 20 pounds
- Occasionally stoops and bends
- Occasionally reaches and grasps with arms and hands
- Work involves possible exposure to laser sources, malodorous vapors, animal allergens and other animal-borne hazards, viruses, experimental microbial agents, low dose radiation, contamination by toxic chemicals and acids and presence of carcinogenic substances
- Long periods of standing and/or sitting
HHMI is an Equal Opportunity Employer.
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