Research Associate II - Huss Lab

Requisition ID: 9797

Job Title: Research Associate II - Huss Lab

Position Summary and Key Responsibilities

A Research Associate II is sought to investigate mechanisms of metabolic and growth regulation in heart and skeletal muscle by orphan nuclear receptor transcription factors. The laboratory integrates molecular, cellular and whole animal approaches to identify novel pathways of estrogen-related receptor (ERR) mediated gene regulation and to determine their effects on energy metabolism in muscle-specific KO and overexpression mouse models.

Candidates should have experience in fundamental experimental design and data analysis. The position will involve manipulation and analysis of mouse models, including in vivo metabolic phenotypes (eg. high fat feeding, treadmill exercise, glucose/insulin tolerance) and ex vivo analysis of muscle metabolism using Seahorse flux analyzer and histochemistry. Therefore, animal/mouse model experience is required and experience with cryostat sectioning is preferred. Candidate will assist PI with general management of small animal colony (PCR-based genotyping and census/recordkeeping). Preferred candidate will also have experience with basic molecular biology techniques (eg. real-time PCR, gel electrophoresis, western analysis) and cell culture. With more experienced candidates there will be opportunity for independent projects under supervision of PI.

Essential function (candidate will be trained in specialized methods):

  • Manipulation and analysis of mouse models, including in vivo metabolic phenotypes (eg. high fat feeding, treadmill exercise, glucose/insulin tolerance)
  • Analysis of muscle metabolism ex vivo using Seahorse flux analyzer and histochemistry. Candidate will be trained in methods.
  • Performs a variety of routine and non-routine laboratory research experiments. Records and validates results for accuracy and completeness.
  • Troubleshoots procedures if experiment results are unexpected or unsatisfactory and recommends alternative approaches.
  • Responsible for accurately recording work performed including results and methodology. Calculates, compiles and analyzes data obtained from work performed.
  • Expected to read relevant literature assigned by PI and related to techniques used in the lab.
  • Ensures that all laboratory activities are carried out in compliance with safety guidelines in handling, storage and disposal of bio-hazardous, infectious and toxic material.

Follows established City of Hope and department policies, procedures, objectives, performance improvement, attendance, safety, environmental, and infection control guidelines, including adherence to the workplace Code of Conduct and Compliance Plan. Practices a high level of integrity and honesty in maintaining confidentiality.

Performs other related duties as assigned or requested.

Minimum Education and Skills Required for Consideration:

  • Bachelor's degree (B.S. or B.A.) (with 2+ years practical lab experience) or Masters degree (with 1+ year practical lab experience) preferred
  • Candidate must have rodent handling (mouse model) experience
  • Experience with histology and cryostat sectioning is preferred
  • Candidates must have solid background in basic laboratory skills, ability to workindependently and willingness to work in a team-oriented manner.
  • Candidate must have meticulous work habits, be detail-oriented and have strongcommunication skills.
  • Basic experience with data analysis and graphics software programs.

About City of Hope

City of Hope, an innovative biomedical research, treatment and educational institution with over 4000 employees, is dedicated to the prevention and cure of cancer and other life-threatening diseases and guided by a compassionate, patient-centered philosophy. Founded in 1913 and headquartered in Duarte, California, City of Hope is a remarkable non-profit institution, where compassion and advanced care go hand-in-hand with excellence in clinical and scientific research. City of Hope is a National Cancer Institute designated Comprehensive Cancer Center and a founding member of the National Comprehensive Cancer Network, an alliance of the nation's 20 leading cancer centers that develops and institutes standards of care for cancer treatment.

City of Hope is an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, religion, color national origin, sex, age, status as a protected veteran, or status as a qualified individual with disability.


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